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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 21, 2026

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
05:48

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry

Published on: September 5, 2014

On-line mass spectrometry: membrane inlet sampling.

Katrin Beckmann1, Johannes Messinger, Murray Ronald Badger

  • 1School of Biology, Australian National University, Canberra, ACT 0200, Australia.

Photosynthesis Research
|August 5, 2009
PubMed
Summary

Membrane inlet mass spectrometry (MIMS) analyzes stable isotopes in gases, offering insights into plant photosynthesis and respiration. This technique tracks reaction flux and isotopic exchange for various biological and inorganic systems.

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Area of Science:

  • Plant Biology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Stable isotope analysis is crucial for understanding gas exchange in biological systems.
  • Membrane inlet mass spectrometry (MIMS) offers a sensitive method for gas analysis.

Purpose of the Study:

  • To review the application of MIMS in studying plant photosynthesis and respiration.
  • To highlight MIMS's utility in analyzing stable isotope distribution of gases.

Main Methods:

  • Utilizing a gas-permeable membrane for direct gas entry into the mass spectrometer.
  • Employing isotopic labeling to differentiate competing reaction pathways.
  • Measuring isotopic exchange rates for chemical kinetics.

Main Results:

  • MIMS effectively analyzes volatile and atmospheric gases.
  • The technique differentiates oxygen evolution and uptake in photosynthesis.
  • MIMS quantifies isotopic exchange rates in biological and inorganic systems, including water splitting.

Conclusions:

  • MIMS is a simple, durable, and sensitive technique for analyzing gas-related processes in plants.
  • Its applicability is growing for diverse research in photosynthesis, respiration, and inorganic reactions.