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Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

Diagnostic testing in suspected fluoroquinolone hypersensitivity.

C S Seitz¹, E B Bröcker, A Trautmann

¹Department of Dermatology, Venereology and Allergology, University of Würzburg, Germany.

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology

|September 9, 2009

Summary

This summary is machine-generated.

Clinical history often overestimates fluoroquinolone hypersensitivity. Oral challenge tests are essential for accurately diagnosing or excluding hypersensitivity to these widely used antibiotics.

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Area of Science:

Pharmacology
Clinical Immunology
Infectious Diseases

Background:

Fluoroquinolone antibiotics possess broad-spectrum activity, good bioavailability, and tissue penetration.
Despite their benefits, fluoroquinolones can induce hypersensitivity reactions, including immediate and delayed types.

Purpose of the Study:

To evaluate diagnostic testing for suspected fluoroquinolone-induced hypersensitivity.
To determine the reliability of history, skin tests, and in vitro assays in identifying hypersensitivity.

Main Methods:

Retrospective analysis of 101 patients with suspected fluoroquinolone hypersensitivity.
Utilized standardized skin testing and oral challenges.
In vitro tests were performed for patients with anaphylaxis symptoms.

Main Results:

Hypersensitivity was excluded in 71 patients via tolerated oral challenges.
Six patients developed anaphylaxis during challenge tests; IgE-mediated mechanisms were not confirmed in vitro.
Patch testing was consistently negative, though challenge tests induced rash in two patients.

Conclusions:

Patient history alone significantly overestimates fluoroquinolone hypersensitivity.
Skin and in vitro tests show limited utility in identifying hypersensitive individuals.
Oral challenge tests are crucial for definitive diagnosis of fluoroquinolone hypersensitivity.