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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides
09:58

An Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides

Published on: November 29, 2016

Protein detection using oligonucleotide probes.

Aya Shibata1, Hiroshi Abe, Kazuhiro Furukawa

  • 1RIKEN Advanced Science Institute, Wako, Saitama 351-0198, Japan.

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed
Summary
This summary is machine-generated.

We created a novel nucleic acid fluorescence probe for detecting proteins. This method uses an aptamer that splits into two parts, enabling sensitive detection of target proteins like the arginine-rich motif peptide.

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Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
06:52

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Protein detection is crucial in various scientific fields.
  • Existing methods may have limitations in sensitivity or specificity.
  • Nucleic acid-based probes offer a promising alternative for molecular detection.

Purpose of the Study:

  • To develop a new nucleic acid-based fluorescence probe for protein detection.
  • To demonstrate the probe's ability to detect the arginine-rich motif peptide (ARM).
  • To establish a sensitive fluorescence readout for protein presence.

Main Methods:

  • Designed a fluorescence probe utilizing an aptamer split into two components.
  • Conjugated the aptamer fragments with a chemically reactive fluorogenic compound.
  • Utilized protein-dependent association to trigger a fluorescence-generating chemical reaction.
  • Detected target protein presence via fluorescence spectroscopy at 450 nm.

Main Results:

  • The probe successfully detected the arginine-rich motif peptide in solution.
  • A significant increase in fluorescence signal at 450 nm was observed in the presence of the peptide.
  • No significant fluorescence increase occurred in the absence of the target peptide, indicating high specificity.
  • The oligonucleotide-based probe demonstrated effective protein detection capabilities.

Conclusions:

  • A novel nucleic acid-based fluorescence probe for protein detection was successfully developed.
  • The probe system exhibits high sensitivity and specificity for target proteins.
  • This method provides a versatile platform for detecting various proteins using fluorescence readouts.