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Updated: Jun 20, 2026

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Handy and prompt DNA separation using PNA with internal disulfide bond
Yuichiro Aiba1, Makoto Komiyama
1Research Center for Advanced Science and Technology, The University of Tokyo, Tokyo 153-8904, Japan.
Abstract:
PNA (peptide nucleic acid) is a DNA analogue which has a peptide backbone. PNA is very useful as a recognition probe because of its immensely high affinity to DNA. In this study, we used PNA as a DNA separation tool. Furthermore, we introduced a cleavable disulfide bond into main chain of PNA in order to facilitate the removal of PNA from PNA/DNA duplex. This disulfide bond was readily cleaved by various reducing agents and then the resultant two short PNA fragments could not form stable PNA/DNA duplex anymore. Accordingly, a desired DNA fragment was picked up from the DNA mixtures and readily recovered by the reductive treatment.
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