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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
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Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

A selective and sensitive detection of SNP between rice cultivars by using DNA photoligation

Yoshinaga Yoshimura1, Hajime Okada, Tomoko Ohtake

  • 1School of Materials Science, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa 923-1292, Japan.

Nucleic Acids Symposium Series (2004)
|September 15, 2009
PubMed

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Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...