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Detection of Modified Forms of Cytosine Using Sensitive Immunohistochemistry
Published on: August 16, 2016
A photochemical detection of methylcytosine by using hydrophobic interaction
Takehiro Ami1, Masayuki Ogino, Yuuta Taya
1School of Materials Science, Japan Advanced Institute of Science and Technnology, Nomi, Ishikawa 923-1292, Japan.
Nucleic Acids Symposium Series (2004)
|September 15, 2009
Abstract:
In this paper, the nonenzymatic detection of 5-methylcytosine with high selectivity is described. We present a new methylation detection method by using template-directed photoligation through 5-cyanovinyl-2'-deoxyuridine ((C)U). Significantly, the photoligation yield of the 5-methylcytosine case was approximately 9.1-fold higher than that in the case of cytosine.
