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Updated: Jun 19, 2026
Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues
Published on: May 8, 2016
[The study of changes on NKT cells of experimental autoimmune encephalomyelitis (EAE) mice]
Qing Ouyang1, Kun Chen, Xi Wang
1Department of Stomatology Lochus 6, the Fourth Military Medical University, Xi'an 710032, China. ouyangqing-hellen@163.com
Aim:
To observe the changes of the number of NKT cells in spleens and livers of induced model of experimental autoimmune encephalomyelitis (EAE), and to study the role NKT cells play in the immunoregulation of EAE.
Methods:
C57BL/6 mice were immunized with MOG (35-55) peptide and received clinical evaluation daily. The mice were sacrificed at the fastigium and the splenic and hepatic lymphocytes were isolated. The changes of NKT cells in normal and EAE C57BL/6 mice were detected by flow cytometry.
Results:
The percent of NKT cells in lymphocytes of different organs of EAE model were greater decreased than in that of normal mice. The percent of NKT cells in splenic lymphocytes of normal mice was 2.22+/-0.14, while that in EAE mice was 1.94+/-0.07 (P<0.05). The percent of NKT cells in hepatic lymphocytes of normal mice was 5.52+/-2.17, while that in EAE mice was 2.67+/-1.41 (P<0.05).
Conclusion:
The proliferation of splenic and hepatic NKT cells in C57BL/6 mice are inhibited in EAE model, which may indicate that the immune function conducted by NKT cell is down regulated in EAE mice.
