Two-color trapped-particle optical microscopy
Related Experiment Video
Updated: Jun 19, 2026

Optical Trap Loading of Dielectric Microparticles In Air
Published on: February 5, 2017
Abstract:
We demonstrate a method for nonintrusive scanned near-field optical microscopy. The microscope utilizes an optical trap to position accurately a 50-100-nm-diameter lithium niobate particle. The infrared trapping beam is frequency doubled in the particle, resulting in a visible microscopic optical probe. By separation of the trapping and detection wavelengths, objects that are transparent in the infrared (e.g., biological) may be positioned close to the particle, resulting in high resolution. The current experimental resolution is limited to approximately 500 nm by the properties of the test objects. The theoretical resolution is less than 100 nm.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy

