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Updated: Jun 19, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
Published on: February 14, 2022
A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests
Mickael Lafond1, Nicolas Vidal, Yves Letourneux
1UPCAM iSm2, Case 342, Université Paul Cézanne, Av. Escadrille Normandie Niémen, 13397 Marseille cedex 13, France.
Introduction:
Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method.
Methods:
The susceptibility of two bacterial and two fungal strains to a 6h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods.
Results:
Three rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones.
Discussion:
The development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing.
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