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THE PREPARATION OF SECTIONS OF GUINEA PIG LIVER FOR ELECTRON MICROSCOPY
1Laboratories of The Rockefeller Institute for Medical Research, and The Research Laboratories, Interchemical Corporation, New York.
A new method prepares guinea pig liver cells for electron microscopy. High-resolution imaging reveals distinct particulate and fibrous components within the cell ground substance.
Area of Science:
- Cell Biology
- Microscopy Techniques
Background:
- Understanding cellular ultrastructure is crucial for cell biology.
- Electron microscopy offers high resolution for visualizing cellular components.
Purpose of the Study:
- To describe a method for preparing guinea pig liver cells for electron microscopy.
- To identify components within the cellular ground substance using electron microscopy.
Main Methods:
- Fixation of guinea pig liver cell sections.
- Preparation of samples for electron microscopy.
- High-resolution electron microscopic imaging.
Main Results:
- Successful preparation of guinea pig liver cells for electron microscopy.
- Identification of two distinct components in the cell ground substance.
- Characterization of components as particulate and fibrous.
Conclusions:
- The described method enables detailed ultrastructural analysis of liver cells.
- The cellular ground substance contains both particulate and fibrous elements.
- Further research can explore the function of these newly identified components.
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01:20Preparation of Samples for Electron Microscopy
01:03Fixation and Sectioning
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...