Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lipid Network Crosslinked Hydrogels: Controlling Material Dynamics Across Multiple Length Scales Through Lipid Movement.

bioRxiv : the preprint server for biology·2026
Same author

Physiological Buffer Selection Alters the Mechanics of Hydrogels with Hydrazone Cross-Links.

Biomacromolecules·2026
Same author

Scientific breakthrough versus successful startup: teaching scientists to navigate bench to business.

Trends in biotechnology·2026
Same author

A diffusion-based 3D printing strategy to fabricate self-supporting, perfusable networks.

BMC methods·2026
Same author

Hydrogel-imposed boundary conditions guide single-lumen neuroepithelial morphogenesis.

bioRxiv : the preprint server for biology·2026
Same author

Polarized Distribution of Lipid Droplets with Long Acyl Chains and Unsaturation are Hallmarks of Human Intestinal Enteroid Differentiation.

Analytical chemistry·2025

Related Experiment Video

Updated: Jun 17, 2026

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

Two-component protein-engineered physical hydrogels for cell encapsulation.

Cheryl T S Wong Po Foo1, Ji Seok Lee, Widya Mulyasasmita

  • 1Materials Science and Engineering and Bioengineering, Stanford University, 476 Lomita Mall, Stanford, CA 94305, USA.

Proceedings of the National Academy of Sciences of the United States of America
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed novel mixing-induced, two-component hydrogels for cell encapsulation. This strategy avoids harsh environmental triggers, enabling reproducible cell culture and tissue engineering applications.

More Related Videos

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

Related Experiment Videos

Last Updated: Jun 17, 2026

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering
09:37

Cellular Encapsulation in 3D Hydrogels for Tissue Engineering

Published on: October 26, 2009

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

Synthesis of an Intein-mediated Artificial Protein Hydrogel
15:06

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Published on: January 27, 2014

Area of Science:

  • Biomaterials Science
  • Protein Engineering
  • Cell Biology

Background:

  • Conventional hydrogel formation requires environmental triggers (pH, temperature, ionic strength) that can harm cells.
  • These triggers complicate reproducible cell encapsulation for clinical applications.

Purpose of the Study:

  • To develop a novel cell encapsulation method using molecular-recognition hydrogels without environmental triggers.
  • To demonstrate the utility of WW and proline-rich domains in protein-engineered hydrogels for reproducible cell encapsulation.

Main Methods:

  • Developed two-component hydrogels based on WW and proline-rich peptide domains that self-assemble upon mixing.
  • Investigated the impact of domain frequency and association energy on hydrogel viscoelasticity.
  • Encapsulated various cell types, including PC-12, endothelial cells, and neural stem cells.

Main Results:

  • Achieved tunable hydrogel viscoelasticity (9-50 Pa) through molecular design.
  • Demonstrated that the hydrogels are shear-thinning, injectable, and self-healing.
  • Showcased stable three-dimensional cultures of neural stem cells with self-renewal, differentiation, and neurite sprouting.

Conclusions:

  • Mixing-induced, two-component hydrogels offer a gentle and reproducible method for cell encapsulation.
  • These protein-engineered hydrogels are suitable for advanced cell culture and tissue engineering, particularly for neural stem cells.