Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Bending of Members Made of Several Materials01:11

Bending of Members Made of Several Materials

574
In analyzing a structural member composed of two different materials with identical cross-sectional areas, it is crucial to understand how their distinct elastic properties affect the member's response under load. The analysis involves assessing stress and strain distributions using the transformed section concept, which accounts for variations in material properties.
Hooke's Law determines stress in each material, stating that stress is proportional to strain but varies due to each material's...
574
Members Made of Elastoplastic Material01:19

Members Made of Elastoplastic Material

384
The behavior of elastoplastic materials under bending stresses, particularly in structural members with rectangular cross-sections, is crucial for predicting material responses and understanding failure modes. Initially, when a bending moment is applied, the stress distribution across the section follows Hooke's Law and is linear and elastic. This distribution means the stress increases from the neutral axis to the maximum at the outer fibers, up to the elastic limit.
As the bending moment...
384
Genetic Material01:20

Genetic Material

3.3K
Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.
3.3K
Bending of Material: Problem Solving01:09

Bending of Material: Problem Solving

504
In this lesson, determine the ratio of the maximum bending moments applied to two metal pipes, given that both pipes can withstand a maximum stress of 100 MPa. Both pipes have an outer radius of 1.8 cm. Pipe A has an inner radius of 1.5 cm, and Pipe B has an inner radius of 1 cm. The ratio of the maximum bending moment applied to two metallic pipes, each with a different inner and outer radius, is determined by considering their dimensions. The inner radius of the first pipe is 1.5 cm, and for...
504
Circular Shafts - Elastoplastic Materials01:24

Circular Shafts - Elastoplastic Materials

476
The study of solid circular shafts under stress shows that within the elastic limit, stress increases directly to the distance from the shaft's center. This relationship holds until the shaft reaches a critical point of stress, beyond which it begins to yield, marking the transition from elastic to plastic deformation. At this crucial juncture, the maximum torque the shaft can endure without permanent deformation is determined, signifying the limit of its elastic behavior.
As torque on the...
476
Precipitation Processes01:12

Precipitation Processes

5.9K
The experimental conditions in a gravimetric analysis should be optimized to maximize the particle size and purity of the obtained precipitate. Ideally, the concentration of the precipitating reagent should be low with effective stirring to maintain low relative supersaturation for the growth of large crystals. In homogeneous precipitation, the precipitant is slowly generated by a chemical reaction in the solution to avoid local reagent excesses. For example, urea decomposes gradually to...
5.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autopsy series of 68 cases dying before and during the 1918 influenza pandemic peak.

Proceedings of the National Academy of Sciences of the United States of America·2011
Same author

Tissue microarrays: construction and uses.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Overview of flow cytometry and fluorescent probes for flow cytometry.

Methods in molecular biology (Clifton, N.J.)·2009
Same author

Tissue disaggregation.

Methods in molecular biology (Clifton, N.J.)·2009
Same author

Indirect immunofluorescent labeling of viable cells.

Methods in molecular biology (Clifton, N.J.)·2009
Same author

Indirect immunofluorescent labeling of fixed cells.

Methods in molecular biology (Clifton, N.J.)·2009
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Jan 26, 2026

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials
08:52

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Published on: July 7, 2017

10.7K

Deparaffinization and processing of pathologic material.

Robert E Cunningham1

  • 1Department of Biophysics, Armed Forces Institute of Pathology, Washington, DC, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 17, 2009
PubMed
Summary
This summary is machine-generated.

This study presents methods for isolating nuclei from paraffin-embedded tissues. Combining cell sorting with polymerase chain reaction (PCR) allows for detailed analysis of nuclear proteins and proliferation factors.

More Related Videos

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
09:05

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials

Published on: May 15, 2015

15.3K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.3K

Related Experiment Videos

Last Updated: Jan 26, 2026

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials
08:52

High-resolution Episcopic Microscopy HREM - Simple and Robust Protocols for Processing and Visualizing Organic Materials

Published on: July 7, 2017

10.7K
Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
09:05

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials

Published on: May 15, 2015

15.3K
Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy
07:37

Revealing Dynamic Processes of Materials in Liquids Using Liquid Cell Transmission Electron Microscopy

Published on: December 20, 2012

13.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Paraffin-embedded tissues are a valuable resource for retrospective studies.
  • Isolation of intact nuclei is crucial for accurate molecular analysis.
  • Investigating nuclear proteins and proliferation factors requires robust methodologies.

Purpose of the Study:

  • To describe methods for preparing whole nuclei from paraffin-embedded tissues.
  • To demonstrate the utility of combining cell sorting with polymerase chain reaction (PCR) for nuclear analysis.
  • To enable the investigation of cellular parameters like nuclear proteins and proliferation factors.

Main Methods:

  • Development of protocols for whole nucleus isolation from paraffin-embedded tissue sections.
  • Application of fluorescence-activated cell sorting (FACS) for isolating specific nuclei populations.
  • Utilizing polymerase chain reaction (PCR) techniques for downstream molecular analysis of sorted nuclei.

Main Results:

  • Successful isolation of intact nuclei from archival paraffin-embedded tissues.
  • Demonstration of cell sorting efficacy in separating distinct nuclear populations.
  • Validation of PCR-based analysis for nuclear proteins and proliferation markers in sorted nuclei.

Conclusions:

  • The described methods provide a reliable approach for nuclear isolation from paraffin-embedded tissues.
  • The integration of cell sorting and PCR offers a powerful tool for investigating nuclear characteristics.
  • This technique facilitates deeper understanding of cellular processes and disease mechanisms.