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The ID23-2 structural biology microfocus beamline at the ESRF
David Flot1, Trevor Mairs, Thierry Giraud
1European Molecular Biology Laboratory, 6 rue Jules Horowitz, BP 181, 38042 Grenoble, France. flot@esrf.fr
Journal of Synchrotron Radiation
|December 24, 2009
Summary
The ID23-2 beamline offers a reliable microfocus X-ray beam for macromolecular crystallography. This dedicated beamline, operational since 2005, simplifies microfocus experiments for researchers.
Area of Science:
- Structural Biology
- Crystallography
- Synchrotron Radiation
Background:
- The European Synchrotron Radiation Facility (ESRF) constructed ID23-1, a tunable MAD-capable beamline, in 2004.
- ID23-2 is the second phase, a monochromatic microfocus beamline for macromolecular crystallography.
Purpose of the Study:
- To describe the design of the ID23-2 beamline.
- To detail considerations for microfocus X-ray beams.
- To summarize initial operational results.
Main Methods:
- Utilizes a single-bounce silicon (111) monochromator.
- Employs Kirkpatrick-Baez geometry mirrors for X-ray focusing.
- Integrates standard ESRF automated macromolecular crystallography environment (hardware and software).
Main Results:
- ID23-2 provides a reliable, user-friendly microfocus X-ray beam.
- The beamline commenced operation in November 2005.
- Facilitates rapid user familiarization with the microfocus environment.
Conclusions:
- ID23-2 is the first beamline dedicated to microfocus macromolecular crystallography.
- The beamline design successfully addresses the needs of microfocus experiments.
- Early operations demonstrate the beamline's effectiveness and ease of use.
