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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

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Related Experiment Video

Updated: Jun 17, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Autophagy regulation by p53.

Maria Chiara Maiuri1, Lorenzo Galluzzi, Eugenia Morselli

  • 1INSERM, U848, F-94805 Villejuif, France.

Current Opinion in Cell Biology
|January 2, 2010
PubMed
Summary
This summary is machine-generated.

The tumor suppressor protein p53 (also known as p53) regulates autophagy, a cellular process crucial for health and disease. Its function depends on location, impacting cancer progression.

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siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

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Last Updated: Jun 17, 2026

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase
09:51

Cell-Based Drug Screening for Inhibitors of Autophagy Related 4B Cysteine Peptidase

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09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Oncology

Background:

  • Autophagy is a fundamental catabolic process involved in physiological functions and diseases like cancer.
  • The regulation of autophagy involves complex signaling pathways that are not fully understood.
  • The tumor suppressor protein p53 plays a role in modulating autophagy.

Purpose of the Study:

  • To review the dual role of p53 in regulating autophagy.
  • To highlight the significance of p53's subcellular localization in autophagy modulation.
  • To focus on p53's recently discovered function as a master regulator of autophagy.

Main Methods:

  • Literature review of studies investigating p53 and autophagy.
  • Analysis of signaling cascades involving p53.
  • Examination of p53's function based on its subcellular localization (nuclear vs. cytoplasmic).

Main Results:

  • p53 exhibits dual regulation of autophagy based on its location.
  • Nuclear p53 transactivates genes that promote apoptosis, cell cycle arrest, and autophagy.
  • Cytoplasmic p53 can promote cell death at mitochondria and repress autophagy through unclear mechanisms.

Conclusions:

  • p53 is a key regulator of autophagy, influencing its activity through distinct mechanisms depending on its cellular compartment.
  • Understanding p53's role in autophagy is critical for comprehending its involvement in cancer and other pathologies.
  • Further research is needed to fully elucidate the mechanisms by which cytoplasmic p53 represses autophagy.