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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...

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Related Experiment Video

Updated: Jun 17, 2026

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
07:35

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

Current technological challenges in biomarker discovery and validation.

Peter L Horvatovich1, Rainer Bischoff

  • 1Analytical Biochemistry, Department of Pharmacy, University of Groningen, A. Deusinglaan 1, 9713 AV Groningen, The Netherlands. P.L.Horvatovich@rug.nl

European Journal of Mass Spectrometry (Chichester, England)
|January 13, 2010
PubMed
Summary
This summary is machine-generated.

This review details biomarker discovery using liquid chromatography-mass spectrometry (LC-MS). It emphasizes collaboration and rigorous methods to ensure reliable biomarker identification and reduce false positives/negatives.

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Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
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Last Updated: Jun 17, 2026

Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances
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Selecting Multiple Biomarker Subsets with Similarly Effective Binary Classification Performances

Published on: October 11, 2018

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies
07:20

Dried Blood Spot Collection of Health Biomarkers to Maximize Participation in Population Studies

Published on: January 28, 2014

Area of Science:

  • Biomarker discovery
  • Analytical chemistry
  • Mass spectrometry

Background:

  • Biomarker discovery is crucial for disease diagnosis and treatment.
  • Liquid chromatography-mass spectrometry (LC-MS) is a key technology in this field.
  • Effective biomarker discovery requires interdisciplinary collaboration.

Purpose of the Study:

  • To provide an overview of challenges in biomarker discovery studies.
  • To highlight the role and optimization of LC-MS methods.
  • To discuss strategies for reducing variance and improving reliability.

Main Methods:

  • Experimental design including sampling, collection, and storage.
  • Validation of quantitative profiling platforms (LC-MS).
  • Data processing, statistical analysis, and validation workflows.

Main Results:

  • Biomarker candidates require rigorous validation in independent cohorts.
  • LC-tandem mass spectrometry is increasingly used for targeted validation.
  • Minimizing sources of variance is critical for accurate results.

Conclusions:

  • Successful biomarker discovery relies on careful planning and execution.
  • Robust validation strategies are essential to confirm biomarker utility.
  • Reducing false positives and negatives enhances the clinical relevance of biomarkers.