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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...

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Related Experiment Video

Updated: Jun 16, 2026

Indoor Experimental Assessment of the Efficiency and Irradiance Spot of the Achromatic Doublet on Glass (ADG) Fresnel Lens for Concentrating Photovoltaics
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Spectrophotometer linearity testing using the double-aperture method.

K D Mielenz, K L Eckerle

    Applied Optics
    |February 2, 2010
    PubMed
    Summary
    This summary is machine-generated.

    The double-aperture method accurately corrects spectrophotometer nonlinearity. This technique achieves high precision, essential for reliable transmittance measurements.

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    Area of Science:

    • Spectrophotometry
    • Optical Metrology

    Background:

    • Spectrophotometers require precise nonlinearity correction for accurate transmittance measurements.
    • Existing methods may have limitations in achieving the required precision.

    Purpose of the Study:

    • To determine the nonlinearity correction for a new spectrophotometer.
    • To assess the precision and error sources of the double-aperture method.

    Main Methods:

    • Utilized the double-aperture method for nonlinearity correction.
    • Analyzed random and systematic errors associated with the method.
    • Developed techniques for high-precision additive nonlinearity correction.

    Main Results:

    • Achieved a precision of +/-4 x 10(-5) transmittance units.
    • The nonlinearity correction was found to be independent of source polarization.
    • Correction showed slight dependence on wavelength and was free from interference errors.

    Conclusions:

    • The double-aperture method provides a precise nonlinearity correction for spectrophotometers.
    • The method is robust, with minimal dependence on polarization and interference.
    • Wavelength dependence requires consideration for specific applications.