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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

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Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
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Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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UV–Vis Spectrum01:30

UV–Vis Spectrum

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When light passes through a substance, a portion of the light is absorbed while the remaining light is reflected or transmitted. If the molecule absorbs light between the wavelengths of 180–400 nm range, the UV spectrum is obtained, and if it absorbs light in the 400–780 nm wavelength range, the visible spectrum is obtained.     
The UV–Vis spectrum of a molecule is the plot of its absorbance versus wavelength. The plot is drawn by taking molar...
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UV–Vis Spectroscopy: Beer–Lambert Law01:09

UV–Vis Spectroscopy: Beer–Lambert Law

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The Beer-Lambert law describes the relationship between absorbance and concentration, which combines the principles established by scientists Johann Heinrich Lambert and August Beer. Lambert's law states that when light passes through a medium, the loss in intensity is directly proportional to the original intensity and the path length of the light. Beer's law proposed that the transmittance of a solution remains constant if the product of concentration and path length is constant. The modern...
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Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

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An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
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Updated: Dec 26, 2025

Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
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Adaptation of a High-Accuracy Spectrophotometer for Ultraviolet Work.

K D Mielenz1, R Mavrodineanu1, E D Cehelnik1

  • 1Institute for Materials Research, National Bureau of Standards, Washington, D.C. 20234.

Journal of Research of the National Bureau of Standards. Section A, Physics and Chemistry
|March 20, 2020
PubMed
Summary
This summary is machine-generated.

A modified spectrophotometer now accurately measures ultraviolet light without performance loss. This advancement enables new Standard Reference Materials development and materials research in the 200-300 nm range.

Keywords:
Averaging sphereUV achromatsdeuterium arc lampfluorescent wavelength convertergratingspectro photometrystandard reference materialsultravioletvisible

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Area of Science:

  • Spectroscopy
  • Optical Physics
  • Materials Science

Background:

  • Existing spectrophotometers designed for visible wavelengths often lack performance in the ultraviolet spectrum.
  • Accurate UV measurements are crucial for developing new materials and standards.

Purpose of the Study:

  • To adapt a visible-wavelength spectrophotometer for high-accuracy ultraviolet (UV) measurements.
  • To maintain the instrument's original performance while extending its spectral range.

Main Methods:

  • Integration of a stable deuterium arc source for UV emission.
  • Inclusion of an efficient averaging sphere with a fluorescent wavelength converter.
  • Addition of a new grating and achromatic optics for the sample compartment.

Main Results:

  • The modified spectrophotometer successfully performs measurements in the 200-300 nm UV region.
  • No degradation in the instrument's original performance was observed.
  • The system provides high accuracy for UV spectral analysis.

Conclusions:

  • The upgraded spectrophotometer is suitable for developing new Standard Reference Materials (SRMs) in the UV range.
  • The instrument will facilitate advanced materials research requiring precise UV measurements.
  • This modification expands the utility of existing spectrophotometer technology.