Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intermittent parathyroid hormone employs autonomous and non-autonomous mechanisms to drive osteogenesis from Ebf3-expressing skeletal progenitor cells.

bioRxiv : the preprint server for biology·2026
Same author

Using biomaterial-based 3D in vitro cancer models to solve current clinical problems.

British journal of cancer·2026
Same author

Towards noninvasive white blood cell count.

Biomedical optics express·2026
Same author

Developing scalable woven textile-based scaffolds for tubular tissues with Core-Sheath nanofibre yarns and tuneable weave architectures.

Frontiers in bioengineering and biotechnology·2025
Same author

Gene editing in "cell villages" enables exploring disease-relevant mutations in many genetic backgrounds.

bioRxiv : the preprint server for biology·2025
Same author

Mesenchymal thymic niche cells enable regeneration of the adult thymus and T cell immunity.

Nature biotechnology·2025
Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

Engineered vascularized bone grafts.

Olga Tsigkou1, Irina Pomerantseva, Joel A Spencer

  • 1Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Proceedings of the National Academy of Sciences of the United States of America
|February 6, 2010
PubMed
Summary
This summary is machine-generated.

This study developed a two-stage protocol for creating vascularized bone grafts using human mesenchymal stem cells (hMSCs) and endothelial cells. The method successfully generated stable, functional microvascular networks essential for tissue-engineered bone grafts.

More Related Videos

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
11:22

Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor

Published on: June 14, 2011

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method
09:38

Scaling of Engineered Vascular Grafts Using 3D Printed Guides and the Ring Stacking Method

Published on: March 27, 2017

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Current clinical protocols for bone grafts use bone marrow for scaffold seeding, but core hypoxia limits graft quality.
  • Enhancing scaffold remodeling and fusion requires improved vascularization within bone grafts.
  • Developing methods to create extensive vascular networks is crucial for higher-quality tissue-engineered bone grafts.

Purpose of the Study:

  • To develop a two-stage protocol for generating vascularized bone grafts using human mesenchymal stem cells (hMSCs) and endothelial cells.
  • To investigate the role of hMSCs in forming stable vascular networks within bone scaffolds.
  • To assess the maturation and host integration of engineered vascular networks in bone grafts.

Main Methods:

  • A mouse model was used to implement a two-stage protocol for vascularized bone graft generation.
  • Human bone marrow-derived mesenchymal stem cells (hMSCs) and umbilical cord-derived endothelial cells were utilized.
  • The formation, stability, and maturation of vascular networks were analyzed in vitro and in vivo.

Main Results:

  • Endothelial cells formed tube-like structures and networks within the bone scaffold within 4-7 days post-implantation.
  • hMSCs were critical for vascular stability, with undifferentiated hMSCs yielding more extensive vasculature than those with a smooth muscle phenotype.
  • Vascular anastomosis occurred by day 11, with networks maturing by 4 weeks and initiating scaffold mineralization. Host remodeling of vasculature was observed by 5 months.

Conclusions:

  • Clinically relevant progenitor cells can generate functional microvascular networks for tissue-engineered bone grafts.
  • The developed protocol successfully creates vascularized bone grafts with potential for enhanced remodeling and fusion.
  • This approach offers a promising strategy for improving the quality and integration of bone graft substitutes.