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Updated: Jun 15, 2026

Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice
Published on: March 23, 2019
Glycinergic interneurons in the respiratory network of the rhythmic slice preparation
Stefan M Winter1, Jens Fresemann, Christian Schnell
1Abt. Neuro- und Sinnesphysiologie und DFG Forschungszentrum für Molekularphysiologie des Gehirns, Zentrum Physiologie und Pathophysiologie, Georg-August-Universität, 37073 Göttingen, Germany. shuelsm2@uni-goettingen.de
Abstract:
The neuronal network in the pre-Bötzinger Complex is the key element of respiratory rhythm generation. Isolated in a slice preparation, the pre-Bötzinger Complex network is still able to generate its inspiratory activity. Although the mechanism of rhythm generation in principle relies on glutamatergic neurons, interestingly we found that glycinergic neurons represent a major portion of all inspiratory neurons in the slice preparation.
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