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Related Experiment Video

Updated: Jun 14, 2026

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
11:03

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

[Visualization of selective transsynaptic neural pathways using a genetic method].

Yoshihiro Yoshihara1

  • 1Laboratory for Neurobiology of Synapse, RIKEN Brain Science Institute, 2-1 Hirosawa, Wakoshi, Saitama 351-0198, Japan.

Brain and Nerve = Shinkei Kenkyu No Shinpo
|March 20, 2010
PubMed
Summary
This summary is machine-generated.

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Wheat germ agglutinin (WGA) cDNA transgenes enable visualization of neuronal circuits by crossing synapses. This review details the WGA transgene technology, its applications, progress, and potential pitfalls in neuroscience research.

Area of Science:

  • Neuroscience
  • Molecular Biology

Context:

  • Neuronal circuit wiring dictates nervous system function.
  • Understanding neural connectivity is crucial for deciphering brain functions.

Purpose:

  • To review the Wheat Germ Agglutinin (WGA) cDNA transgene technology for neuronal circuit analysis.
  • To summarize the principles, applications, progress, and limitations of this neuroanatomical tracing method.

Summary:

  • The WGA transgene technology utilizes the transsynaptic properties of WGA to label connected neurons.
  • Developed in 1999, this method has been refined and applied to various neural systems.
  • Potential misinterpretations of results highlight the need for careful application and understanding of caveats.

Impact:

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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Published on: May 2, 2010

Related Experiment Videos

Last Updated: Jun 14, 2026

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain
11:03

Conditional Genetic Transsynaptic Tracing in the Embryonic Mouse Brain

Published on: December 22, 2014

Viral Tracing of Genetically Defined Neural Circuitry
13:06

Viral Tracing of Genetically Defined Neural Circuitry

Published on: October 17, 2012

Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation
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Deciphering Axonal Pathways of Genetically Defined Groups of Neurons in the Chick Neural Tube Utilizing in ovo Electroporation

Published on: May 2, 2010

  • Provides a valuable tool for mapping selective and functional neural pathways.
  • Facilitates detailed analysis of complex neural systems.
  • Highlights advancements and critical considerations for neuroanatomical research using WGA transgenes.