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Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity

Published on: October 28, 2013

Fluorescent cell-based sensing approaches for toxicity testing.

Michael Fritzsche¹, Carl-Fredrik Mandenius

¹Division of Biotechnology/IFM, Linköping University, 581 83 Linköping, Sweden.

Analytical and Bioanalytical Chemistry

|April 1, 2010

Summary

This summary is machine-generated.

Fluorimetric cell-based sensing offers advanced toxicity testing for chemicals and drugs. These fluorescence methods show promise for high-throughput screening in drug discovery and safety pharmacology.

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Published on: December 25, 2016

Area of Science:

Biotechnology
Analytical Chemistry
Toxicology

Background:

Cell-based sensing methods are increasingly vital for evaluating the toxicity of pharmaceuticals, pathogens, and environmental pollutants.
Fluorimetric techniques offer sensitive and specific detection capabilities for these applications.

Purpose of the Study:

To provide a comprehensive review of current fluorimetric cell-based sensing approaches for toxicity testing.
To highlight recent advancements and discuss the underlying mechanisms and fluorophore roles.

Main Methods:

Review of various fluorimetric methods including fluorescent indicators, membrane integrity assays, fluorescence labeling, fluorescent protein expression, and autofluorescence analysis.
Analysis of the mechanisms and fluorophore contributions in each method.

Main Results:

Fluorimetric methods offer advantages over traditional non-fluorescence assays.
Several approaches demonstrate significant potential for high-throughput screening applications.

Conclusions:

Fluorimetric cell-based sensing is a powerful tool for diverse toxicity assessments.
These methods are well-suited for applications in drug discovery and safety pharmacology due to their efficiency and sensitivity.