Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.7K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.7K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

21.6K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
21.6K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.6K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.6K
Stem Cell Culture01:17

Stem Cell Culture

5.0K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differential Effects of Retinal Fatty Acids Under Oxidative Stress Reveal DHA's Susceptibility to Ferroptosis and Polarity Disruption in iPSC Derived RPE Cells.

Stem cell reviews and reports·2026
Same author

Human-Induced Pluripotent Stem Cell-Derived Definitive Endoderm Bulk Up and Hepatic Differentiation.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells for the Study of Macular Degeneration.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Utility of Induced Pluripotent Stem Cell-Based Microphysiological Systems for Drug Development and Testing.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Evaluating the Effect of Drug Compounds on Cardiac Spheroids Using the Cardiac Cell Outgrowth Assay in a Microphysiological System.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Correction: Clinical Classification of Cancer Cachexia: Phenotypic Correlates in Human Skeletal Muscle.

PloS one·2024
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 9, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.2K

Cell-Based Assays Using Derived Human-Induced Pluripotent Cells in Drug Discovery and Development.

James A Ross1, Carl-Fredrik Mandenius2

  • 1Tissue Injury & Repair Group, Chancellor's Building, University of Edinburgh, Edinburgh, UK. j.a.ross@ed.ac.uk.

Methods in Molecular Biology (Clifton, N.J.)
|April 30, 2025
PubMed
Summary
This summary is machine-generated.

Human-induced pluripotent stem cells offer significant advantages for drug development, enabling disease modeling and screening. Their use in creating diverse cell types for assays and ADME testing is revolutionizing pharmaceutical research.

Keywords:
Differentiation protocolsDisease modelsDrug screeningInduced pluripotent celliPSC

More Related Videos

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.5K
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

2.7K

Related Experiment Videos

Last Updated: May 9, 2025

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery
12:40

Using Human Induced Pluripotent Stem Cell-derived Hepatocyte-like Cells for Drug Discovery

Published on: May 19, 2018

10.2K
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.5K
Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
10:30

Technical Applications of Microelectrode Array and Patch Clamp Recordings on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Published on: August 4, 2022

2.7K

Area of Science:

  • Biotechnology
  • Stem Cell Research
  • Drug Discovery

Background:

  • Human-induced pluripotent stem cells (hiPSCs) have advanced significantly in cell differentiation and assay development.
  • The application of hiPSCs in drug development is a rapidly evolving field with notable potential.
  • Understanding the benefits and limitations of hiPSCs is crucial for their effective implementation.

Purpose of the Study:

  • To introduce the field of induced pluripotent stem cells for drug development.
  • To discuss the rationale, advantages, and disadvantages of using hiPSCs in pharmaceutical research.
  • To highlight the potential of hiPSCs in creating patient-specific disease models and screening platforms.

Main Methods:

  • Review of existing methodologies and protocols for hiPSC differentiation and application.
  • General perspective discussion of various techniques and experiences.
  • Comparative analysis with similar protocols documented in scientific literature.

Main Results:

  • hiPSCs facilitate the differentiation of specific cell types for various applications.
  • Stratified cell sources from diverse patient groups can be generated using hiPSCs.
  • Organ and tissue cells derived from hiPSCs are valuable for disease models, screening assays, and ADME testing.

Conclusions:

  • Induced pluripotent stem cells represent a powerful tool for advancing drug discovery and development.
  • The use of hiPSCs enables personalized medicine approaches through patient-specific cell models.
  • Further exploration of hiPSC methodologies promises to enhance the efficiency and accuracy of pharmaceutical research.