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Updated: Jun 14, 2026
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Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Published on: May 9, 2019
[Proliferation markers used in histological studies].
Morfologiia (Saint Petersburg, Russia)
|April 3, 2010
Summary
This review covers methods for studying cell proliferation using markers like 5-bromodeoxyuridine and Ki-67. It details proteins crucial for cell cycle control and their roles in identifying proliferating cells.
Area of Science:
- Cell Biology
- Molecular Biology
Context:
- Accurate assessment of cell proliferative activity is crucial in biological research and diagnostics.
- Recent discoveries have identified key proteins involved in cell cycle regulation that can serve as proliferation markers.
Purpose:
- To review current immunocytochemical methods for studying cell proliferative activity.
- To characterize specific protein markers used for identifying proliferating cells.
Summary:
- The paper details immunocytochemical techniques for detecting 5-bromodeoxyuridine (BrdU), Proliferating Cell Nuclear Antigen (PCNA), Ki-67, Flap Endonuclease 1 (FEN-1), phosphorylated histone H3 (H3P), and cyclins.
- It presents data on the function of these proteins in regulating the cell cycle.
Impact:
- Provides a comprehensive overview of established and emerging markers for cell proliferation.
- Facilitates the selection of appropriate methods for quantifying cell division in various biological contexts.
