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Raman Spectroscopy: Overview

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

Raman Spectroscopy Instrumentation: Overview

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Updated: Jun 14, 2026

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

Published on: November 17, 2023

Quantifying resonant Raman cross sections with SERS.

Stefan A Meyer¹, Eric C Le Ru, Pablo G Etchegoin

¹The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical and Physical Sciences, Victoria University of Wellington, PO Box 600, Wellington, New Zealand.

The Journal of Physical Chemistry. A

|April 10, 2010

Summary

This summary is machine-generated.

This study introduces a surface-enhanced Raman scattering (SERS) method to measure resonance Raman cross sections for dyes, overcoming fluorescence interference. The technique effectively quantifies Raman signals from fluorescent molecules.

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Fabrication of polydimethylsiloxane (PDMS)-Based Flexible Surface-Enhanced Raman Scattering (SERS) Substrate for Ultrasensitive Detection

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Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

Published on: June 16, 2023

Area of Science:

Chemical Spectroscopy
Molecular Physics
Analytical Chemistry

Background:

Resonance Raman cross sections are crucial for understanding molecular light-matter interactions.
High fluorescence quantum yields in dyes often mask their Raman spectra, hindering accurate measurements.
Conventional Raman spectroscopy struggles with strongly fluorescent samples.

Purpose of the Study:

To develop a novel method for estimating resonance Raman cross sections of dyes.
To overcome the challenge of fluorescence interference in Raman spectroscopy.
To utilize surface-enhanced Raman scattering (SERS) for improved Raman signal detection.

Main Methods:

Employing surface-enhanced Raman scattering (SERS) to induce fluorescence quenching.
Utilizing the fluorescence quenching effect in SERS to enable Raman signal detection.
Describing the principles and limitations of the proposed SERS-based method.

Main Results:

Successfully estimated resonance Raman differential cross sections for Rhodamine 6G.
Demonstrated the method's applicability across seven different visible excitation wavelengths.
Validated the SERS approach for quantifying Raman signals from highly fluorescent dyes.

Conclusions:

The proposed SERS method effectively overcomes fluorescence interference in Raman spectroscopy.
This technique provides a viable route for measuring resonance Raman cross sections of fluorescent molecules.
The method offers a valuable tool for the spectroscopic characterization of dyes.