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Development of novel fibrinogen nanoparticles by two-step co-acervation method
N Sanoj Rejinold1, M Muthunarayanan, N Deepa
1Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, India.
International Journal of Biological Macromolecules
|April 13, 2010
Summary
Fibrinogen nanoparticles were successfully synthesized and characterized, showing non-toxicity and structural integrity. These novel nanoparticles demonstrate significant cellular uptake, indicating their potential as effective drug delivery carriers.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Protein Chemistry
Background:
- Fibrinogen is a key protein in blood coagulation.
- Nanoparticle drug delivery systems offer enhanced therapeutic potential.
- Developing biocompatible carriers is crucial for effective drug delivery.
Purpose of the Study:
- To synthesize and characterize fibrinogen nanoparticles.
- To evaluate the non-toxicity and structural integrity of fibrinogen nanoparticles.
- To investigate the potential of fibrinogen nanoparticles as drug delivery carriers.
Main Methods:
- Two-step co-acervation method with calcium chloride cross-linking.
- Characterization using DLS, SEM, AFM, FT-IR, TG, DTA, and XRD.
- Cytotoxicity assessment via MTT assay on NIH3T3, L929, and SKBR3 cell lines.
- Fluorescence spectroscopy to confirm protein structure retention.
- Cellular uptake studies using Rhodamine 123-conjugated nanoparticles and fluorescence microscopy.
Main Results:
- Fibrinogen nanoparticles were successfully prepared and characterized.
- Nanoparticles exhibited non-toxic properties across tested cell lines.
- Protein structure was maintained within the nanoparticle formulation.
- Significant cellular uptake and retention of nanoparticles were observed in L929 cells.
Conclusions:
- Fibrinogen nanoparticles are biocompatible and structurally stable.
- These nanoparticles show promising cellular internalization capabilities.
- Fibrinogen nanoparticles represent a potential superior drug delivery system.
