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A colorimetric method for detection of K-ras codon 12 point mutations in DNA extracted from tissue and peripheral
Robert A Ollar1, Avram M Cooperman, Michael E Wayne
1Department of Surgery, St. Vincent's Hospital Manhattan, New York, NY 10011, USA. rao48@yahoo.com
Abstract:
Molecular-based methods to monitor point mutations require special and expensive equipment unavailable in most hospitals. Colorimetric-based analysis is an ideal platform for K-ras codon 12 gene point mutations because it uses commonly found hospital equipment. The colorimetric assay is sensitive and specific, detecting mutated DNA levels as low as 1% in a wild-type background. Paired genomic DNA extracts of fixed tissue and cellular fractions of peripheral blood are more sensitive and accurate than unpaired samplings. This approach has the potential to improve K-ras point mutation scans as well as to detect micrometastases in circulating tumor cells.
