[Multiphoton microscopy and in vivo tomography in dermatologic imaging]

Area of Science:

• Biomedical Optics
• Dermatology
• Medical Imaging

Context:

• Multiphoton microscopy (MPM) and in vivo multiphoton tomography (MPT) provide non-invasive, subcellular resolution imaging.
• These techniques utilize biogenic fluorophores and second harmonic generation (SHG) for high-quality tissue and cell evaluation.
• MPT has been specifically advanced for in vivo diagnostics of skin diseases.

Purpose:

• To review the capabilities of multiphoton-based diagnostics for evaluating transcutaneous metabolism.
• To explore multiphoton techniques for assessing physiological and pathological changes in the dermal fiber network.
• To demonstrate the application of visual biopsy using MPM/MPT for diagnosing dermal and epidermal disorders.

Summary:

• MPM and MPT enable detailed visualization of cellular and extracellular structures, comparable to histology.
• The techniques allow for assessment of the dermal fiber network and transcutaneous metabolic processes.
• Applications include morphological classification of skin tumors and diagnosis of inflammatory or allergic skin lesions.

Impact:

• Enhances non-invasive diagnostic capabilities in dermatology and biomedical research.
• Facilitates early detection and characterization of skin diseases.
• Supports both clinical diagnostics and fundamental scientific investigations at a subcellular level.