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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Jun 13, 2026

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Label-free cell separation and sorting in microfluidic systems.

Daniel R Gossett¹, Westbrook M Weaver, Albert J Mach

¹Department of Bioengineering, University of California Los Angeles, 420 Westwood Plaza, 5121 Engineering V, Box 951600, Los Angeles, CA 90095-1600, USA.

Analytical and Bioanalytical Chemistry

|April 27, 2010

Summary

This summary is machine-generated.

This review explores label-free microfluidic cell separation techniques, focusing on intrinsic cell properties like size and electrical polarizability. It highlights recent advancements and proposes metrics for comparing these promising technologies for broader accessibility.

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Published on: November 23, 2015

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

Cell separation and sorting are critical in research, diagnostics, and therapeutics.
There is a growing need for methods that avoid biochemical labels, utilizing intrinsic cell properties instead.

Purpose of the Study:

To review label-free microfluidic techniques for cell population discrimination and fractionation.
To analyze separation modes, recent developments, and application prospects of these technologies.
To evaluate technologies from a perspective considering inertial effects and propose performance metrics.

Main Methods:

Review of microfluidic systems for label-free cell separation.
Analysis of intrinsic biomarkers including size, electrical polarizability, and hydrodynamic properties.
Focus on inertial effects and development of general performance metrics for technology comparison.

Main Results:

Microfluidic systems offer precise single-cell handling and integration with analytical tools.
Various label-free techniques utilizing intrinsic cell properties have been developed.
Proposed performance metrics facilitate easier comparison of different microfluidic cell separation technologies.

Conclusions:

Label-free microfluidic cell separation is a rapidly advancing field with significant potential.
Further development is needed to enhance the accessibility and applicability of these technologies.
Inertial effects play a crucial role in separation efficiency and performance.