Related Experiment Video
Updated: Jun 13, 2026
Generation of Monoclonal Antibodies Against Natural Products
Published on: April 6, 2019
[Preparation and characterization of specific monoclonal antibodies against HER2]
Hong-qin Liu1, Dong-mei Wang, Hao Qu
1Union Stem Cell &Gene Engineering Co., Ltd., Tianjin 300384, China.
Aim:
To prepare and characterize the specific monoclonal antibodies (mAbs) that can recognize the native conformation of HER2 so as to guide the clinical application of Herceptin.
Methods:
Hybridomas were generated by the fusion with the NS1 myeloma cell and spleen cell, which were from mice immunized by HER2 recombinant protein. After cell fusion, ELISA, Yeast cell base ELISA and immunohistochemistry were used to screening clones respectively.
Results:
Eight clones that could react with SK-Br-3 and yeast displaying HER2 were selected from 168 clones which were positive in initial screening by ELISA.
Conclusion:
Successfully prepared 8 mAbs that can recognize the native conformation of HER2, which should provide useful reagent for personalized therapy against breast cancer with Herceptin.
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