Related Experiment Videos
Plateletpheresis with the COBE spectra single needle access option
B C McLeod1, R McKenna, A Viernes
1Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
Journal of Clinical Apheresis
|January 1, 1991
Summary
Single needle plateletpheresis using the COBE Spectra device yields comparable platelet quantity and quality to the dual needle method. This modification offers a viable alternative for apheresis procedures, ensuring effective platelet collection.
Area of Science:
- Hematology
- Apheresis Technology
Background:
- Plateletpheresis is a critical procedure for collecting platelets.
- The COBE Spectra device is commonly used for apheresis.
- Single needle procedures can potentially improve patient comfort and reduce complications.
Purpose of the Study:
- To evaluate the feasibility and efficacy of a modified single needle plateletpheresis protocol using the COBE Spectra.
- To compare the yield and quality of platelets collected via single versus dual needle protocols.
Main Methods:
- A modified single needle plateletpheresis protocol was developed for the COBE Spectra, incorporating a reservoir bag.
- Paired donations were performed on 10 subjects comparing single and dual needle procedures.
- Platelet yield, leukocyte levels, hemolysis, pH, morphology, and beta-thromboglobulin levels were assessed.
Main Results:
- No evidence of hemolysis was observed with either protocol.
- Mean platelet yields were statistically similar between single (3.31 +/- 1.31 x 10(11)) and dual needle (3.61 +/- 1.47 x 10(11)) procedures.
- Leukocyte levels, storage pH, morphology scores, and beta-thromboglobulin levels showed no significant differences between the two protocols.
Conclusions:
- The single needle plateletpheresis modification with the COBE Spectra effectively collects platelets comparable in quantity and quality to the standard dual needle protocol.
- This single needle approach represents a safe and effective alternative for platelet apheresis.