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Updated: Jun 13, 2026

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues
Published on: February 17, 2017
[Peculiarities of surface-active trehalose mycolates synthesis of Rhodococcus erythropolis EK-1]
Abstract:
It has been established that activity of trehalosephosphate synthase (key enzyme of trehalosemycolates synthesis) under cultivation of Rhodococcus erythropolis EK-1 on the medium with n-hexadecane was 10 times higher, and amount of synthesized surface-active substances 4 times higher as compared to the growing of EK-1 strain on ethanol. Introduction of 0.2% of fumarate and 0.1% of citrate to the cultivation medium of R. erythropolis EK-1 with 2% of n-hexadecane, as well as 0.2% of fumarate to the medium with 2% of ethanol was accompanied by intensification of synthesis of surface-active trehalosemycolates, that is evidenced by the 1.3-1.6-fold increase of the amount of synthesized surface-active substances under such conditions, and by 3-5-fold increase of activity of phosphoenolpyruvate synthetase (key enzyme ofgluconeogenesis) and trehalosephosphate synthase. The paper is presented in Ukrainian.
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