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Related Experiment Video

Updated: Jun 12, 2026

Impression Cytology of the Lid Wiper Area

Impression Cytology of the Lid Wiper Area

Published on: August 9, 2016

Control specimens for immunocytochemistry in liquid-based cytology.

T Hansen¹, H Pedersen, V Brauner

¹Department of Pathology, Sygehus Sønderjylland, Sønderborg, Denmark. tovelhansen@gmail.com

Cytopathology : Official Journal of the British Society for Clinical Cytology

|May 21, 2010

Summary

Developing control specimens for immunocytochemistry (ICC) using liquid-based cytology (LBC) is now feasible. Fresh tissue and body fluid samples can be processed into slides and stored for at least 40 days without losing antigenicity.

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Area of Science:

Cytopathology
Immunohistochemistry
Molecular Diagnostics

Background:

Immunohistochemistry (IHC) controls are standard, but suitable immunocytochemistry (ICC) controls are challenging to establish.
Existing literature lacks comprehensive methods for preparing ICC control specimens.

Purpose of the Study:

To develop and validate a method for selecting, collecting, processing, and storing control specimens for ICC using liquid-based cytology (LBC).

Main Methods:

Utilized 21 antibodies targeting cellular components from tonsils, serous fluids, and bronchial washings.
Collected tonsil specimens via brushing into SurePath™ vials; fixed serous fluids and bronchial washings in CytoRich Red™.
Prepared unstained SurePath™ slides, stored them at 5°C for up to 40 days, and performed ICC.

Main Results:

Unstained SurePath™ slides demonstrated positive reactivity with relevant antibodies.
Stored slides maintained staining intensity for up to 40 days, indicating preserved antigenicity.

Conclusions:

Body fluid and cell-suspension specimens from fresh tissues are effective as ICC controls, individually or as mixtures.
Dry, unstained slides can be stored refrigerated for at least 40 days without compromising antigenicity for ICC.