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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: Jun 12, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
11:26

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

High-throughput DNA sequencing--concepts and limitations.

Martin Kircher1, Janet Kelso

  • 1Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany.

Bioessays : News and Reviews in Molecular, Cellular and Developmental Biology
|May 21, 2010
PubMed
Summary
This summary is machine-generated.

High-throughput DNA sequencing offers rapid, low-cost genomics, enabling deep sequencing for many researchers. Understanding platform-specific error profiles and limitations is crucial for experimental design and future technological development.

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Last Updated: Jun 12, 2026

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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Published on: June 3, 2019

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
10:00

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Published on: May 23, 2018

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10:34

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Published on: March 15, 2019

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Recent advances in DNA sequencing technologies have dramatically reduced costs and increased speed.
  • High-throughput sequencing enables deep transcriptome and whole genome sequencing for broader research access.

Purpose of the Study:

  • To review and compare current high-throughput DNA sequencing technologies.
  • To analyze the error profiles, limitations, and selection criteria for different sequencing platforms.
  • To discuss future developments and remaining challenges in DNA sequencing.

Main Methods:

  • Review of current high-throughput DNA sequencing technologies.
  • Comparative analysis of error rates, speed, and cost.
  • Discussion of experimental considerations for platform selection.

Main Results:

  • New sequencing platforms offer significant cost and time reductions.
  • Platforms exhibit diverse error profiles and limitations compared to older technologies.
  • Informed selection of sequencing platforms is critical for experimental success.

Conclusions:

  • Understanding the nuances of high-throughput sequencing platforms is essential for researchers.
  • Future developments aim to overcome current limitations and enhance sequencing capabilities.
  • Continued innovation is needed to address remaining challenges in the field.