Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Formation of the Platelet Plug

Formation of the Platelet Plug

The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...

Structure and Function of Platelets

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Glycemic control and blood product quality of Canadian blood donors with diabetes.

Transfusion·2026

Same author

Manufacturing stresses do not differentially impact red blood cells from donors with diabetes.

Vox sanguinis·2025

Same author

Hemocompatibility of cyclodextrin-coated magnetic nanoparticles under uremic conditions.

Carbohydrate polymers·2025

Same author

Investigation on the Influence of Protein Corona and Platelet Adhesion on Storage Bag Surface on the Platelet Storage Lesion.

Advanced healthcare materials·2025

Same author

Characterization and Hemocompatibility of α, β, and γ Cyclodextrin-Modified Magnetic Nano-Adsorbents.

International journal of molecular sciences·2024

Same author

Genetic engineering of transfusable platelets with mRNA-lipid nanoparticles is compatible with blood banking practices.

Blood·2024

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

The platelet storage lesion.

Dana V Devine¹, Katherine Serrano

¹Research and Development, Canadian Blood Services, 2350 Health Sciences Mall, Vancouver, Canada. dana.devine@blood.ca

Clinics in Laboratory Medicine

|June 2, 2010

Summary

Platelet storage lesion, a quality decline during storage, is now the main challenge for extending platelet concentrate shelf life. New technologies aim to mitigate this storage lesion for better transfusion outcomes.

Area of Science:

Hematology
Transfusion Medicine
Biotechnology

Background:

Platelet storage lesion (PSL) describes the deterioration of platelet quality during storage, assessed via metabolic, functional, and morphologic assays.
Advances in pathogen reduction technologies and bacterial contamination testing have shifted focus to PSL as the primary obstacle for extending the shelf life of platelet concentrates.

Purpose of the Study:

To discuss how platelet production methods contribute to PSL.
To review established and novel methods for assessing platelet quality.
To highlight the clinical significance of PSL for transfusion outcomes.

Main Methods:

Discussion of platelet production impacts on PSL.
Review of current and emerging in vitro assays for platelet quality assessment.

More Related Videos

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Related Experiment Videos

Last Updated: Jun 12, 2026

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Comprehensive Analysis of Procoagulant Platelets Exhibiting Features of Necrosis, Apoptosis and Platelet Activation

Published on: May 23, 2025

Analysis of novel storage technologies, including platelet additive solutions and pathogen inactivation.

Main Results:

Platelet production methods significantly influence the development of PSL.
Various assays provide insights into different aspects of platelet quality degradation.
Novel technologies like additive solutions and pathogen reduction show promise in mitigating PSL.

Conclusions:

Understanding and addressing the platelet storage lesion is crucial for improving platelet concentrate quality and efficacy.
Emerging technologies offer potential solutions to enhance platelet shelf life and clinical outcomes.
Further research into platelet storage lesion mitigation is essential for advancing transfusion medicine.