[Determination of four components in vitamin C Yinqiao tablets using reversed-phase high performance liquid...

A reversed-phase high performance liquid chromatographic (RP-HPLC) method with dual wavelength detection was developed for the determination of vitamin C, paracetamol, chlorphenamine maleate and chlorogenic acid in Vitamin C Yinqiao Tablets. The separation was performed on a Sinochrom ODS-BP column with the mobile phase consisting of 0.05 mol/L KH2PO4 (pH 3.0, containing 1% triethylamine and acetonitrile (75 : 25, v/v). The detection wavelength was set at 260 nm (lambda 1) and 326 nm (lambda 2). The method linearities were wide and the average recoveries were more than 99.4%. The relative standard deviations were less than 1.8% (n = 5). This method is convenient, rapid and accurate. It is readily applied to the quality controls in the production process of Vitamin C Yinqiao Tablets.