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Lensless Fluorescent Microscopy on a Chip
Published on: August 17, 2011
Integrated microfluidic biophotonic chip for laser induced fluorescence detection.
Arvind Chandrasekaran1, Muthukumaran Packirisamy
1Optical Bio Microsystems Laboratory, Department of Mechanical Engineering, Concordia University, Montreal, QC, Canada. achandra@alcor.concordia.ca
Biomedical Microdevices
|June 22, 2010
Summary
This study introduces an integrated microfluidic biophotonic chip for laser-induced fluorescence detection. The device enables high-throughput analysis of chemical and biological specimens with enhanced sensitivity.
Area of Science:
- Biophotonics
- Microfluidics
- Analytical Chemistry
Background:
- Lab-on-a-Chip (LOC) and Micro-Total Analysis Systems (µTAS) offer advantages for detecting chemical and biological species.
- Integrated systems are crucial for miniaturized, high-throughput analytical platforms.
Purpose of the Study:
- To propose and demonstrate an integrated microfluidic biophotonic chip for laser-induced fluorescence detection.
- To integrate a Spectrometer-on-Chip (SoC) device with a microfluidic chip for multi-wavelength fluorescence analysis.
Main Methods:
- Fabrication of a Silicon-Polymer hybrid opto-microfluidic chip.
- Integration of laser source input and Spectrometer-on-Chip output fibers with the microfluidic channel.
- Fluorescence detection using Alexafluor 647 tagged antibody particles.
Main Results:
- A robust setup was achieved through fiber integration with the microfluidic channel.
- The device demonstrated suitability for high-throughput detection.
- Successful fluorescence detection was performed using labeled antibody particles.
Conclusions:
- The proposed biophotonic microfluidic device is highly effective for sensitive, high-throughput detection.
- This integrated system advances the capabilities of microfluidic analytical devices.
- The platform shows promise for various chemical and biological sensing applications.
