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Updated: Jun 12, 2026
High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Validated LC-MS (ESI) assay for the simultaneous determination of amitriptyline and its metabolite nortriptyline in
Yuan Shen1, Rong-Hua Zhu, Huan-De Li
1Clinical Pharmacy & Pharmacology Institute, Second Xiangya Hospital, Central South University, Middle Renmin Road 139#, Changsha 410011, Hunan, PR China. sybonnie@163.com
Abstract:
A rapid, sensitive and specific method based on high performance liquid chromatography with electrospray ionization mass spectrometry (HPLC-MS/ESI) has been developed for the simultaneous determination of amitriptyline and nortriptyline in rat plasma. Sample preparation involved liquid-liquid extraction with methyl t-butyl ether after alkalified with 0.5 mol/l NaOH. Chromatographic separation was performed on a XB-C4 column (4.6 mm x 250 mm, 5 microm, Welch Materials) with a mobile phase consisting of 10mM ammonium acetate (0.6 per thousand formic acid)-acetonitrile (60:40, v/v) at a flow rate of 1.0 ml/min. Calibration curves were linear within the ranges of 10-3200 ng/ml for amitriptyline and 10-1000 ng/ml for nortriptyline. This method was successfully applied to the pharmacokinetic study in rats after intravenous injection of amitriptyline hydrochloride.
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