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Related Experiment Video

Updated: Jun 11, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Micropipe flow visualization using digital in-line holographic microscopy.

Nicolas Verrier1, Clément Remacha, Marc Brunel

  • 1Groupe d'Optique et d'Optoélectronique, UMR 6614-CORIA, avenue de l'Université, 76801 Saint-Etienne du Rouvray cédex, France. nicolas.verrier@coria.fr

Optics Express
|July 1, 2010
PubMed
Summary
This summary is machine-generated.

Digital in-line holography visualizes particle motion in microfluidic pipes. This method uses advanced optics to precisely track particles in 3D, improving microflow analysis.

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

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Related Experiment Videos

Last Updated: Jun 11, 2026

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

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Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

  • Optical Engineering
  • Microfluidics
  • Particle Imaging

Background:

  • Particle motion analysis is crucial for understanding microfluidic systems.
  • Traditional methods face challenges in precise 3D localization within confined spaces.

Purpose of the Study:

  • To develop and validate a digital in-line holography technique for visualizing particle motion in cylindrical micropipes.
  • To enhance the axial localization accuracy of particles within microflows.

Main Methods:

  • Utilized digital in-line holography and the generalized Huygens-Fresnel integral with ABCD matrices.
  • Reconstructed holograms using fractional Fourier transformation.
  • Leveraged astigmatism induced by the cylindrical micropipe for 3D region selection.

Main Results:

  • Successfully visualized particle dynamics within a 100-micrometer diameter micropipe.
  • Demonstrated improved axial localization of particles through astigmatism analysis.
  • Presented experimental results and a supporting video of microflow particle motion.

Conclusions:

  • Digital in-line holography offers a robust method for 3D particle tracking in microfluidic devices.
  • The technique effectively overcomes limitations in axial localization within cylindrical geometries.
  • This approach advances the study of microscale fluid dynamics and particle behavior.