Related Experiment Video
Updated: Jun 11, 2026

Subcutaneous Administration of Muscarinic Antagonists and Triple-Immunostaining of the Levator Auris Longus Muscle in Mice
Published on: September 8, 2011
[Antianaphylactic effects of muscarinic antagonists]
Abstract:
The study was carried out on color guinea pigs, in which anaphylactic shock was induced by sensitizing and challenging doses of normal horse serum (HS). Administration of atropine, ipratropium, or ipratropium in combination with neostigmine led to reduction of sensitizing effect of HS: anaphylactic index was decreased up to (2.2 - 2.4) +/- 0.1 arbitrary units (a.u.). Methacine in combination with neostigmine (0.4 +/- 0.01 a.u.) or atropine in combination with galantamine (1.5 +/- 0.3 a.u.) effectively prevented the pathochemical stage of anaphylactic shock. Changes in plasma protein concentrations modified effects of methacine: application of methacine with IgG was protective (0.5 +/- 0.1 a.u.), while CRP abolish the antianaphylactic activity of methacine (3.8 +/- 0.1 a.u.). Guinea pigs which survived shock had a great number of AFC [(880 +/- 22) x 10(6) splenocytes in experimental group and (100 +/- 29) x 10(6) in control) and low concentration of 5-HT in the spleen (at a shock, 0.443 +/- 0.005; basal level, 1.532 +/- 0.004 ng/mg protein). Effective prevention of anaphylactic shock resulted in normalization of B cell activity and 5-HT level in the spleen. Thus, cholinergic drugs modulate the immunological and pathochemical stages of anaphylactic shock.
Related Concept Videos
Cholinergic Antagonists: Pharmacological Actions
Gastrointestinal Effects: Antimuscarinics reduce gut contractions, increase gastric emptying, and slow intestinal transit. They partly inhibit gastric acid secretion...
Antiasthma Drugs: Muscarinic Receptor Antagonists
Antimuscarinic agents compete with ACh for the same binding site on the muscarinic receptors. By binding to these receptors, they inhibit the downstream effects of ACh and block the parasympathetic...
Cholinergic Antagonists: Therapeutic Uses
Respiratory Tract: Ipratropium, aclidinium, and tiotropium treat asthma, chronic bronchitis, and chronic obstructive pulmonary disease (COPD). They protect against bronchoconstriction caused by irritants like cigarette smoke, sulfur dioxide, and ozone. They also help reduce nasopharyngeal secretions in common...
Nondepolarizing (Competitive) Neuromuscular Blockers: Pharmacological Actions
Although all competitive neuromuscular blockers are designed...
Cholinergic Antagonists: Chemistry and Structure-Activity Relationship
Direct-Acting Cholinergic Agonists: Pharmacological Actions
