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Updated: Jun 11, 2026
Generation of Murine Monoclonal Antibodies by Hybridoma Technology
Published on: January 2, 2017
[Generation of monoclonal antibody to GP73 based on antigen epitope]
Xi-ning Yao1, Jing-rui Huo, Ying-fu Liu
1State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing 102206, China. xiningyao3579@yahoo.com.cn
Aim:
Preparation of monoclonal antibody (mAb) against GP73 protein.
Methods:
The N-terminal peptide (AAAERGAVELK) of GP73 protein was displayed on T7 phage, the recombinant phage was amplified and used as the immunogen to immunize mouse to produce antibody. The titer of the antiserum and the positive hybridoma clones which secreted the mAb against GP73 protein were detected by ELISA. The mAb specificity was assayed by ELISA and Western blot.
Results:
The high specificity mAb against GP73 protein was selected from the mouse immunized with the recombinant T7 phages displaying the epitope of GP73 by cell fusion and screening.
Conclusion:
The appropriate protein epitope displayed on T7 phage could be used as alternative antigen to immunize animals to make specific antibody against the corresponding native protein.
