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Investigating Single Molecule Adhesion by Atomic Force Spectroscopy

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Published on: February 27, 2015

Ethanol wet-bonding technique sensitivity assessed by AFM.

E Osorio¹, M Toledano, F S Aguilera

¹Department of Dental Materials, School of Dentistry, University of Granada, Spain. haketa-mvrk@polka.ocn.ne.jp

Journal of Dental Research

|July 28, 2010

Summary

Ethanol wet bonding protocols significantly alter dentin surface properties. Short 100% ethanol rinses decrease roughness and fibril diameter, while progressive ethanol replacement minimizes collagen matrix collapse.

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A Simple Way to Measure Ethanol Sensitivity in Flies

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Published on: February 19, 2011

Area of Science:

Biomaterials Science
Dental Materials Science
Surface Chemistry

Background:

Ethanol wet bonding aims to preserve collagen matrix integrity for resin infiltration.
Short ethanol dehydration protocols may compromise dentin surface structure.
Maintaining surface roughness, fibril diameter, and interfibrillar spaces is crucial for adhesive bonding.

Purpose of the Study:

To test the hypothesis that ethanol dehydration protocols do not affect acid-etched dentin surface roughness, fibril diameter, and interfibrillar spaces.
To compare the effects of different ethanol rinsing durations and concentrations on dentin surface morphology.

Main Methods:

Human dentin surfaces were acid-etched and water-rinsed.
Tested protocols included water-rinse (control), 1-min 100% ethanol, 5-min 100% ethanol, and progressive ethanol replacement (50-100%).
Atomic force microscopy (AFM) evaluated surface roughness, fibril diameter, and interfibrillar spaces. Statistical analysis used one-way ANOVA and Student-Newman-Keuls test.

Main Results:

100% ethanol rinses (1-5 min) significantly reduced dentin roughness and fibril diameter (p < 0.001).
Absolute ethanol caused collagen fibril collapse and shrinkage.
Progressive ethanol replacement protocols resulted in less matrix collapse and fibril shrinkage compared to absolute ethanol rinses.

Conclusions:

Ethanol dehydration protocols significantly impact the surface morphology of acid-etched dentin.
Short-term 100% ethanol application leads to undesirable changes in dentin structure.
Progressive ethanol replacement appears to be a more favorable method for preserving dentin collagen matrix during ethanol wet bonding.