Multiplexed NMR: an automated CapNMR dual-sample probe

Area of Science:

• Analytical Chemistry
• Spectroscopy
• Nuclear Magnetic Resonance

Background:

• Microscale nuclear magnetic resonance (CapNMR) probes offer high sensitivity for analyzing small sample volumes.
• Current CapNMR technology typically accommodates only one sample at a time, limiting throughput.
• Simultaneous analysis of multiple samples is desirable for increased efficiency in CapNMR applications.

Purpose of the Study:

• To introduce and characterize a novel dual-sample CapNMR probe (DSP).
• To evaluate the performance of the DSP in terms of spectral resolution and signal-to-noise ratio (S/N).
• To demonstrate the flexibility of the DSP for various data acquisition modes, including simultaneous and sequential analysis.

Main Methods:

• Development of a dual-sample CapNMR probe with two independent detection elements.
• Implementation of simultaneous shimming for both samples or flowcell-specific shim set selection.
• Integration with an automation system for dual sample loading and independent flowpaths.

Main Results:

• The DSP achieves equivalent spectral resolution and S/N compared to single-sample CapNMR probes.
• High electrical isolation allows for diverse data acquisition strategies.
• Simultaneous shimming and automated sample loading enhance experimental efficiency.

Conclusions:

• The dual-sample CapNMR probe (DSP) significantly increases throughput for microscale NMR analysis.
• The DSP maintains high analytical performance while enabling simultaneous or efficient sequential sample analysis.
• This technology represents a significant advancement for CapNMR applications requiring high efficiency and flexibility.