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Updated: Jun 10, 2026
Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
Published on: February 25, 2010
[Expression of partial S gene of Hantavirus Z10 in Pichia pastoris]
Han-ping Zhu1, Ping-ping Yao, Xiao-zhao Deng
1The Key Lab of HFRS of Zhejiang Province, The Center for Disease Prevention and Control of Zhejiang Province, Hangzhou, China. hanpingzhu@yahoo.com.cn
Objective:
The purpose of this study is to express partial S gene of Hantavirus Z10.
Methods:
The 300 bp S gene of Z10 strain was synthesized by using a successive PCR method for the optimal expression in Pichia pastoris and subcloned into pMD19-T. The SP300 gene was constructed into pPICZaA and sequenced. The recombinant pPICZaA-SP300 and pPICZaA-S300 was transformed into Pichia with LiCI.
Results:
The recombination Pichia were cultivate, and expressed the SP300 or S300 gene induced in Pichia by methanol.
Conclusion:
The nucleocapsid secreted from the Pichia can be detected by ELISA and WesternBlot.
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