Golgi partitioning controls mitotic entry through Aurora-A kinase.
Angela Persico1, Romina Ines Cervigni, Maria Luisa Barretta
1Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, 66030 Santa Maria Imbaro, Chieti, Italy.
Molecular Biology of the Cell
|September 17, 2010
Summary
Proper Golgi complex inheritance during cell division is monitored by a checkpoint. This checkpoint involves the mitotic kinase Aurora-A, which, when activated, allows cell cycle progression.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- The Golgi complex fragments during mitosis for proper inheritance.
- Inhibition of Golgi fragmentation causes a G2 cell cycle arrest, indicating a Golgi mitotic checkpoint.
- The molecular mechanisms underlying this G2 block are currently unknown.
Purpose of the Study:
- To elucidate the molecular basis of the G2 cell cycle block caused by impaired Golgi fragmentation.
- To identify the key signaling molecules involved in the Golgi mitotic checkpoint.
Main Methods:
- Investigating the role of Golgi fragmentation in cell cycle progression.
- Analyzing the recruitment and activation of the mitotic kinase Aurora-A during mitosis.
- Utilizing cell cycle arrest models and Aurora-A overexpression experiments.
Main Results:
- Golgi fragmentation is linked to the recruitment and activation of Aurora-A at the centrosomes during G2 phase.
- Blocking Golgi partitioning prevents Aurora-A activation, leading to G2 cell cycle arrest.
- Overexpression of Aurora-A can overcome the cell cycle block induced by impaired Golgi partitioning.
Conclusions:
- Aurora-A is a critical effector of the Golgi mitotic checkpoint.
- The Golgi checkpoint ensures correct organelle inheritance by regulating Aurora-A activity.
- These findings reveal a signaling pathway coordinating organelle inheritance and cell duplication.
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