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Updated: Jun 8, 2026
10:05
Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
Published on: October 24, 2018
Intact mass detection, interpretation, and visualization to automate Top-Down proteomics on a large scale
Kenneth R Durbin1, John C Tran, Leonid Zamdborg
1Department of Chemistry, The Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Proteomics
|September 18, 2010
Summary
This study introduces a new automated software pipeline for processing intact protein mass spectrometry data. This pipeline enables large-scale Top-Down proteomics by analyzing whole proteins and their modifications.
Area of Science:
- Proteomics
- Mass Spectrometry
- Bioinformatics
Background:
- High-throughput Top-Down MS requires advanced data processing models.
- Current methods are insufficient for large-scale proteomic analysis of intact proteins.
Purpose of the Study:
- To develop and present an automated software pipeline for Top-Down MS data.
- To capture the full value of intact protein data for proteomic applications.
Main Methods:
- Combined algorithms for MS1 data processing (FT and ion trap).
- Linked intact mass data to fragment ions for database searching (ProSight).
- Developed new modules for proteome-scale data visualization and PTM detection.
Main Results:
- Automated determination of human keratin and tubulin isoforms.
- Selective detection and screening of post-translational modifications (PTMs) like acetylation, phosphorylation, and methylation.
- Demonstrated software functionality on yeast and human cell line data.
Conclusions:
- The developed software pipeline advances the realization of Top-Down MS on a proteomic scale.
- Automated processing of intact protein data is crucial for large-scale proteomic studies.
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