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Updated: Jun 8, 2026
Rescue of Recombinant Newcastle Disease Virus from cDNA
Published on: October 11, 2013
[Transient expression and identification of gene P and NP of NDV LaSota strain in two different cells]
Yuan Zhou1, Run-Qing Jia, Zhi-Ping Teng
1The College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, China.
Objective:
To Construction of P and NP genes eukaryotic expression vectors of Newcastle Disease Virus LaSota strain,study its reverse genetics and functional genome of NDV.
Methods:
P, NP genes were amplified and cloned into pGEM-T easy vector and then subcloned into pcDNA3.1 (+) expression vector respectively, the recombinant plasmids were named pcDNA3.1 (+)-P and pcDNA3.1 (+)-NP, Recombinant plasmids were transfected into 293 and BHK-21 cells respectively and were detected using IE and Western blot analysis.
Results:
Expression of P, NP genes were detected and confirmed by the IE and WB analysis.
Conclusion:
The recombinant eukaryotic plasmids pcDNA3. 1(+)-P, pcDNA3.1 (+)-NP were expressed in 293 and BHK-21 cells successfully. This research may be helpful for further study of reverse genetics and functional genome of NDV.
