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A practical solution-phase synthesis of an antagonistic peptide of TNF-α based on hydrophobic tag strategy

Gegen Tana1, Shingo Kitada, Shuji Fujita

  • 1Laboratory of Bio-organic Chemistry, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.

Chemical Communications (Cambridge, England)
|October 1, 2010
PubMed

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...