Related Experiment Video
Updated: Jun 8, 2026
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
[Screening of breast cancer antigen from a phage display random peptide library]
Wei Zhao1, Lin Meng, Yu-Jing Gao
1Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing 100142, China.
Aim:
to isolate novel breast cancer antigen for developing cancer marker and vaccine, a phage display peptide library was screened with serum antibody from breast cancer patients.
Methods:
a phage display peptide library was screened with serum IgG from six breast cancer patients, and then identified the positive clones by ELISA. According to the sequences of the positive clones, the synthesized oligonucleotides of one small peptide (P15) was cloned into PGEX-4T-1. Finally the fusion protein (GST-P15), which was purified from E.coli. BL21, was checked its binding activity with two kinds of serum antibodies (20 breast cancer patients and 20 normal people) by ELISA.
Results:
38 positive clones which specifically bound with serum IgG from breast cancer patients were screened. Some of these clones had the same DNA sequence. A small peptide (P15) and purified its fusion protein (GST-P15) was obtained successfully. ELISA assay showed GST-P15 had higher affinity with the serum antibody from breast cancer patients than with the normal serum and there was a significant difference (P<0.01).
Conclusion:
the peptide P15 can be recognized specifically by serum antibody from the breast cancer patients and they will be helpful for isolate new cancer antigen from breast cancer.
