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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
[Ethanol production from sweet sorghum stalks by advanced solid state fermentation (ASSF) technology]
Bing Han1, Li Wang, Shizhong Li
1Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China.
Advanced solid state fermentation (ASSF) using Saccharomyces cerevisiae CGMCC1949 significantly boosts fuel ethanol production from sweet sorghum stalks. This cost-effective technology achieves high yields and extends plant operation, proving economically competitive.
Area of Science:
- Biotechnology
- Renewable Energy
- Chemical Engineering
Background:
- Sweet sorghum stalks are a promising lignocellulosic feedstock for bioethanol production.
- Traditional fermentation methods face challenges with efficiency and feedstock storage.
Purpose of the Study:
- To develop and optimize an advanced solid state fermentation (ASSF) technology for fuel ethanol production from sweet sorghum stalks.
- To establish cost-effective feedstock storage solutions.
- To validate the technology at a pilot scale and assess economic viability.
Main Methods:
- Screening and identification of a robust Saccharomyces cerevisiae strain (CGMCC1949).
- Development of ASSF technology with optimized fermentation kinetics and heat-mass transfer.
- Design and scale-up of a rotating drum bioreactor.
- Techno-economic analysis (TEA) using ASPEN software.
Main Results:
- Fermentation time reduced to under 30 hours with an ethanol yield of 92% of theoretical maximum.
- Cost-effective storage of sweet sorghum stalks achieved with <5% sugar loss over 200 days.
- Pilot plant trials demonstrated 91% ethanol yield in a 127 m³ scaled-up bioreactor.
Conclusions:
- The developed ASSF technology offers a highly efficient and economically competitive route for fuel ethanol production from sweet sorghum.
- The integrated approach of feedstock storage, optimized fermentation, and bioreactor design enhances the sustainability of bioethanol production.
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