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FRET Imaging in Three-dimensional Hydrogels
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Construction of a FRET-based ratiometric fluorescent thiol probe

Lingliang Long1, Weiying Lin, Bingbing Chen

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, PR China.

Chemical Communications (Cambridge, England)
|November 13, 2010
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...